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RAS PresidiumВестник Дальневосточного отделения Российской академии наук Vestnik of the Far East Branch of the Russian Academy of Sciences

  • ISSN (Print) 0869-7698
  • ISSN (Online) 3034-5308

Laboratory of Peptide Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences: forty years of research on peptides and proteins of sea anemones

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PII
10.31857/S0869769824030064-1
DOI
10.31857/S0869769824030064
Publication type
Review
Status
Published
Authors
Margarita M. Monastyrnaya
  • Occupation: Doctor of Chemical Sciences, Leading Researcher
  • Affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS
Emma P. Kozlovskaya
  • Occupation: Doctor of Chemical Sciences, Chief Researcher, Professor
  • Affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS
Volume/ Edition
Volume / Issue number 3
Pages
101-120
Abstract
The review briefly describes a research carried out over the past 40 years at the Laboratory of Peptide Chemistry of G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS (LPCh of PIBOC FEB RAS), in collaboration with Russian and foreign colleagues. The results of search, identification, and study of the structure, the biological activity, and the mechanisms of an interaction with the biological targets of peptides and polypeptides produced by the tropical sea anemone Heteractis crispa (=Heteractis magnifica, formerly Radianthus macrodactylus) are discussed. One of the main achievements of LPCh over the past years is the discovery of new structural type 2 neurotoxins, namely, six toxins that were not identified among the representatives of so-called long anemonotoxins in the first decade of foreign research (70–80s of the last century), and among them the first, previously unknown, double-chain neurotoxin. In addition, the presence of several multigene families expressing α-pore-forming toxins (actinoporins), serine protease inhibitors (Kunitz-type peptides), and APETx-like peptides forming the combinatorial libraries of the several dozen of highly homologous family members has been established. Using in silico methods (homologous modeling, alanine mutagenesis, full-atom molecular dynamics (MD) simulation), the spatial structures of the studied peptides and complexes with biological targets were predicted for the first time, and their structure-functional relationships were analyzed. This was the foundation for the further production of recombinant and mutant analogues on the basis of the combinatorial libraries for the purpose of conducting the electrophysiological studies of the mechanisms of their molecular interaction with targets as well as determining the pharmacological potential. In the review the most important results of recent years are presented. They are related to the discovery of analgesic, anti-inflammatory, and antitumor activity in a number of the studied peptides.
Keywords
морские анемоны нейротоксины пептиды Кунитц-типа APETx-подобные пептиды дефензины биологические мишени
Date of publication
15.09.2025
Year of publication
2025
Number of purchasers
0
Views
11

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