Везде

RAS PresidiumВестник Дальневосточного отделения Российской академии наук Vestnik of the Far East Branch of the Russian Academy of Sciences

  • ISSN (Print) 0869-7698
  • ISSN (Online) 3034-5308

INDEL-typing of strains of parahemolytic vibrions in vitro and in silico

You can
PII
10.31857/S0869769824020089-1
DOI
10.31857/S0869769824020089
Publication type
Article
Status
Published
Authors
Olga S. Chemisova
  • Occupation: Candidate of Sciences in Biology, Associate Professor
  • Affiliation: Don State Technical University
Oksana A. Tsyrulina
  • Occupation: Candidate of Sciences in Biology, Senior Researcher
  • Affiliation: The Federal Government Health Institution Rostov-on-Don Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights
Aleksey S. Vodopyanov
  • Occupation: Candidate of Sciences in Medicine, Senior Researcher
  • Affiliation: The Federal Government Health Institution Rostov-on-Don Plague Control Research Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights
Sergey O. Vodopyanov
  • Occupation: Doctor of Sciences in Medicine, Leading Researcher
  • Affiliation: The Federal Government Health Institution Rostov-on-Don Plague Control Researsh Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights
Margarita M. Sagakyants
  • Occupation: Researcher
  • Affiliation: The Federal Government Health Institution Rostov-on-Don Plague Control Researsh Institute of the Federal Service for Surveillance in the Sphere of Consumers Rights
Volume/ Edition
Volume / Issue number 2
Pages
80-89
Abstract
The article presents the results of studying the distribution of Vibrio parahaemolyticus INDEL genotypes in the world and in Russia in vitro and in silico using the previously developed INDEL typing method based on the distribution of alleles of six loci (Vp967, Vp08, Vp619, Vp2256, VpA472, and Vp506). Among 2105 strains of V. parahaemolyticus taken in the study, 30 unique INDEL genotypes were identified, seven of which were identified as major. At the same time, geographical attachment between the genotype of parahaemolytic vibrions and the place of their isolation was revealed. The circulation of 17 INDEL genotypes was detected on the territory of the Russian Federation, the predominant of which was D2, found only in the Russian Federation. As a result, it was found that the proposed INDEL-typing method is a useful tool in conducting epidemiological investigations, which makes it possible to identify phylogenetic relationships between different isolates, which in turn makes it possible to draw conclusions about the sources of splinters of the pathogen to a specific territory.
Keywords
INDEL-типирование INDEL-локусы Vibrio parahaemolyticus парагемолитические вибрионы генотип
Date of publication
15.09.2025
Year of publication
2025
Number of purchasers
0
Views
15

References

  1. 1. Wang R. Z., Huang J. D., Zhang W. et al. Detection and identification of Vibrio parahaemolyticus by multiplex PCR and DNA-DNA hybridization on a microarray // J. Genet. Genomics. 2011. Vol. 38. P. 129–135. DOI: 10.1016/j.jgg.2011.02.002.
  2. 2. Broberg C. A., Calder T. J., Orth K. Vibrio parahaemolyticus cell biology and pathogenicity determinants // Microbes Infect. 2011. Vol. 13. P. 992–1001. DOI: 10.1016/j.micinf.2011.06.013.
  3. 3. Zhang L., Orth K. Virulence determinants for Vibrio parahaemolyticus infection // Curr. Opin. Microbiol. 2013. Vol. 16 (1). P. 70–77. DOI: 10.1016/j.mib.2013.02.002.
  4. 4. Nair G. B., Ramamurthy T., Bhattacharya S. K. et al. Global dissemination of Vibrio parahaemolyticus serotype O3: K6 and its serovariants // Сlin. Microbiol. Rev. 2007. Vol. 20, N1. P. 39–48. DOI: 10.1128/CMR.00025–06.
  5. 5. Yeung P. S., Boor K. J. Epidemiology, pathogenesis, and prevention of foodborne Vibrio parahaemolyticus infections // Foodborne Pathog. Dis. 2004. Vol. 1. P. 74–88. DOI: 10.1089/153531404323143594.
  6. 6. Velazquez-Roman J., Leоn-Sicairos N., Hernаndez-Diaz L., Canizalez-Roman A. Pandemic Vibrio parahaemolyticus O3: K6 on the American continent // Front. Cell. Infect. Microbiol. 2013. Vol. 3. P. 110. DOI: 10.3389/fcimb.2013.00110.
  7. 7. Маслов Д. В. Вспышка галофилеза среди населения города Владивостока Приморского края // Здоровье населения и среда обитания. 1997. № 12 (57). С. 17–21.
  8. 8. Ломов Ю. М., Кудрякова Т. А., Македонова Л. Д. и др. Галофильные вибрионы, обусловившие вспышку пищевой токсикоинфекции во Владивостоке // Журн. микробиол., эпидемиол. и иммунобиол. 2001. № 6. С. 3–7.
  9. 9. Рыковская О. А., Мазрухо А. Б., Смоликова Л. М., Монахова Е. В., Чемисова О. С., Подойницына О. А. и др. Vibrio parahaemolyticus серогруппы О3: К6 – возбудитель вспышек пищевой токсикоинфекции в Приморском крае Российской Федерации // Журн. микробиол., эпидемиол. и иммунобиол. 2013. № 4. С. 57–61.
  10. 10. Водопьянов А. С., Водопьянов С. О., Олейников И. П., Мишанькин Б. Н. INDEL-типирование штаммов Vibrio cholerae // Эпидемиол. и инфекцион. болезни. 2017. Т. 22 (4). С. 195–200. DOI: 10.18821/1560-9529-2017-22-4-195-200.
  11. 11. Liu F., Hu Y., Wang Q. et al. Comparative genomic analysis of Mycobacterium tuberculosis clinical isolates // BMC Genomics. 2014. Vol. 15, N1. Р. 469. DOI: 10.1186/1471-2164-15-469.
  12. 12. Larsson P., Svensson K., Karlsson L. Canonical Insertion-Deletion markers for rapid DNA typing of Francisella tularensis // Emerg. Infect. Diseases. 2007. Vol. 13, N11. Р. 1725–1732. DOI: 10.3201/eid1311.070603.
  13. 13. Чемисова О. С., Водопьянов А. С. Рыковская О. А. и др. Молекулярное типирование штаммов Vibrio parahaemolyticus на основе INDEL-маркеров // Инфекцион. болезни. 2018. № 7 (3). С. 26–30. DOI: 10.24411/2305-3496-2018-13004.
  14. 14. Мишанькин Б. Н., Водопьянов А. С., ЛомовЮ.М. Мультилокусное VNTR-типирование культур холерных вибрионов, выделенных в г. Казань во время вспышки холеры летом 2001 года // Журн. микробиол., эпидемиол. и иммунобиол. 2003. № 6. С. 11–15.
  15. 15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods // Mol. Biol. Evol. 2011.Vol. 28. Р. 2731–2739.
  16. 16. Xiao Chen, Qiaoyun Zhu, Fei Yu, Wen Zhang et al. Serology, virulence and molecular characteristics of Vibrio parahaemolyticus isolated from seafood in Zhejiang province // PLOS One. 2018. Vol. 13, N 10. e0204892. DOI: 10.1371/journal.pone.0204892.
  17. 17. Scallan E., Hoekstra R.M., Angulo F.J. et al. Foodborne Illness Acquired in the United States-Major Pathogens // Emerg. Infect. Diseas. 2011. Vol. 17. Р. 7–15. DOI: 10.3201/eid1701.P11101.
  18. 18. Martinez-Urtaza J., Huapaya B., Gavilan R.G. et al. Emergence of Asiatic Vibrio diseases in South America in phase with El Niño // Epidemiology. 2008. Vol. 19, N 6. Р. 829–837. DOI: 10.1097/EDE.0b013e3181883d43.
  19. 19. Martinez-Urtaza J., Baker-Austin C., Jones J.L. et al. Spread of Pacific Northwest Vibrio parahaemolyticus // N. Engl. J. Med. 2013. Vol. 369. Р. 1573–1574. DOI: 10.1056 /NEJMc1305535.
  20. 20. Kaneko T., Colwell R.R. Ecology of Vibrio parahaemolyticus in Chesapeake Bay // J. Bacteriol. 1973. Vol. 113, N 1. Р. 24–32. DOI: 10.1128/jb.113.1.24-32.1973.
  21. 21. Бузолева Л.С., Летягина А.В., Звягинцев А.Ю., Кашин И.А. Исследование микроорганизмов, поступающих в порт Владивостока с балластными водами судов // Рос. журн. биол. инвазий. 2٠12. № 5 (1). С. 19–31.
  22. 22. Водяницкая С.Ю., Прометной В.И., Телесманич Н.Р., Рыжков Ю.В., Лях О.В., Трут И.В., Иванова Н.Г., Кругликов В.Д., Чемисова О.С. Результаты трехлетнего мониторинга судовых балластных вод в портах Ростовской области // Пробл. особо опасных инфекций. 2014. № 2. С. 108–110. DOI: 10.21055/0370-1069-2014-2-108-110.
QR
Translate

Индексирование

Scopus

Scopus

Scopus

Crossref

Scopus

Higher Attestation Commission

At the Ministry of Education and Science of the Russian Federation

Scopus

Scientific Electronic Library